cd99 flow cytometry miltenyi biotech (Miltenyi Biotec)
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Cd99 Flow Cytometry Miltenyi Biotech, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 2 article reviews
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1) Product Images from "Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B -related neurodevelopmental disorders"
Article Title: Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest formation in ARID1B -related neurodevelopmental disorders
Journal: Nature Communications
doi: 10.1038/s41467-021-26810-x
Figure Legend Snippet: a , b CNCC differentiation was optimized using an ARID1B -wt control line. After 14 days, the cells exhibited the classic CNCC morphology and expressed the CNCC markers. P values are from two-sided Student’s t test. N = 3 biologically independent samples. Error bars display standard errors. c Time-course immunoblot conducted using Control Line-1 during CNCC differentiation shows that ARID1B is active in the first 7 days of the differentiation, with a peak of activity between day 5 and day 7. The ARID1B protein level strongly decreases after day 7. The experiment was repeated twice (the second replicate is shown in Fig. ). Marker bars display kDa. d , e Flow cytometry quantifying the expression of surface markers for pluripotency and CNCC differentiation in Control Line-1 and in the two patient lines. A large cell population is still pluripotent in both patients after 14 days ( d ). The patient lines also show reduced expression of CNCC surface markers after 14 days of differentiation relative to an ARID1B -wt control line at the same time point ( e ).
Techniques Used: Western Blot, Activity Assay, Marker, Flow Cytometry, Expressing
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